This questions and more we answered in our newly published paper in Plant methods by analysing spruce wood and Arabidopsis with three different unmixing methods (VCA, NMF and MCR-ALS):
Multivariate unmixing approaches on Raman images of plant cell walls: new insights or overinterpretation of results?
- Batirtze Prats-Mateu
- Martin Felhofer,
- Anna de Juan and
- Notburga Gierlinger