Raman imaging to elucidate the hidden multicomponent layered structure of plant cuticles : with the guide published in Frontiers of Plant Science we want to share our experience and inspire plant research. Based on the three investigated cuticles (Arabidopsis stem, spruce needle, tomato) we discuss the chemical and structural diversity and validate our findings by comparing models based on our spectroscopic data with the current view of the cuticle. We amend the model by adding the distribution of cinnamic acids and flavonoids within the cuticle layers and their transition to the epidermal layer. https://frontiersin.org/article/10.3389/fpls.2021.793330…
